Get Atlas of Human Pluripotent Stem Cells in Culture PDF

By Lyn Healy, Ludmila Ruban

ISBN-10: 1489975063

ISBN-13: 9781489975065

This lavishly-illustrated, authoritative atlas explores the tricky artwork of culturing human pluripotent stem cells. Twelve chapters – containing greater than 280 colour illustrations – hide a number of subject matters in pluripotent stem mobilephone culturing together with mouse and human fibroblasts, human embryonic stem cells and precipitated pluripotent stem cells, attribute staining styles, and irregular cultures, between others. Atlas of Human Pluripotent Stem Cells in tradition is a entire choice of illustrated innovations complemented by means of informative and academic captions reading what high quality cells seem like and the way they behave in quite a few environments. Examples of excellent cultures are in comparison side-by-side to less-than-perfect and unacceptable examples of human embryonic and precipitated pluripotent stem mobilephone colonies. This particular and thorough atlas is a useful source for researchers, academics, and scholars who're attracted to or operating with stem cellphone culturing.

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The large colony in the top left of image a shows slight differentiation (black arrow), but in general it is a good culture. It is advisable to passage this culture at this stage (both ×4 magnification) b Fig. 6 (a) Two small colonies compacted in the middle, 24 h after passage. (b) The compacted centre of the same colony (a, ×4 magnification; b, ×20) 4 Human Embryonic Stem Cells 39 Fig. 7 Example of a good colony with fresh feeders surrounding it. This colony should be passaged immediately, because it could begin to spontaneously differentiate in the centre if it is left for another 24 h.

19 (a, b) Darkfield microscopy of two colonies that have merged. Colony number 1 is undifferentiated, and the smooth, perfect edge of the compacted colony can be seen. The smaller colony, number 2, is fully differentiated, without smooth edges (both ×4 magnification) 4 Human Embryonic Stem Cells a 45 b c Fig. 20 (a) An undifferentiated colony, with defined edges. The colony contains round, compacted cells with large nucleoli. (b) Higher magnification of the edges of the colony. The cells are elongated, with spaces between them, as is typical for actively proliferating stem cells.

Under phase contrast, these colonies appear to be growing in three dimensions. (b) Two colonies 37 b represent a good undifferentiated colony and would be optimal when expanding undifferentiated cells in a culture system. (b) The same colony at ×10 magnification b merge and spread to form a perfect monolayer, ready for passaging. Both colonies are compacted and ready to passage (a, ×4 magnification; b, ×10) 38 a Fig. 5 (a, b) Relatively good-quality, high-density culture. The colonies are close together and cells within the colonies are compacted.

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Atlas of Human Pluripotent Stem Cells in Culture by Lyn Healy, Ludmila Ruban

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